SUMMARY, EXPLANATION AND LIMITATIONS:
Annexins constitute a group of calcium- and phospholipid-binding proteins whose functions are yet not completely understood. They seem to be involved in metabolic pathways; amongst others in exo- and endocytosis. Annexin A1 (ANXA1) is expressed in the cytoplasmic membrane, often also in the cytoplasm of various cell types. Dreier et al. found the protein in leucocytes of peripheral blood, tissue macrophages and T-cells, in epithelia of the respiratory tract, in superficial cells of non-keratinised squamous epithelium, in mesothelial and urothelial cells, in the Bowman’s capsule, and in peripheral nerve cells. An important use of antibodies against Annexin A1 is the detection of hairy cell leukaemia and its differentiation from other B-cell lymphomas. Moreover, Annexin A1 is described as a useful tool for the characterisation of adenocarcinomas of the oesophagus where the expression of Annexin A1 is considered to be an independent prognostic factor for patient survival. Other possible applications of antibodies to Annexin A1 are the analysis of carcinomas of breast and liver where Annexin A1 is highly expressed. Wang et al. describe Annexin A1 as an effective marker for the detectionof cervical intraepithelial ne oplasias (CIN) and for evaluation of tumour cell differentiation in squamous cell carcinomas of the cervix.
Immunogen: Annexin A1 human.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from hairy cell leukemia, prostate or placenta.
This antibody is designed for the specific localization of human annexin A1 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Immunocytochemical detection of annexin A1 is a simple, highly sensitive and specific for the diagnosis of hairy cell leukemia. It has been reported that annexin A1 is overexpressed in breast carcinomas, hepatocellular carcinoma, pancreas carcinoma and pituitary adenomas and down regulated in esophageal carcinoma, endometrial carcinoma and prostate carcinoma.