SUMMARY, EXPLANATION AND LIMITATIONS:
The Bcl-6 proto-oncogene encodes for a zinc finger protein of about 92 – 98 kDa. This protein binds to nuclear DNA and acts as a transcriptional repressor. The expression of Bcl-6 protein in general is ubiquitous and, therefore, small amounts of this protein are detectable in a lot of cell types. However, strong Bcl-6 expression is only found in B-cells and skeletal muscles. In B-cells of germinal centres high expression levels are detectable but not in precursor cells, mantle zone cells, and plasma cells. Germ centre B-cell derived neoplasms like Burkitt´s lymphoma, follicular lymphoma, and diffuse large cell lymphoma are showing high expression levels too. The analysis of Bcl-6 expression is useful to distinguish proliferating centres in B-cells (bcl6-/bcl2+) from trapped germinal centres with mantle cell lymphoma (bcl6+/bcl2-). The identification of neoplasmic cells in cases of nodular Hodkin´s lymphoma which is characterised by a predominance of lymphocytes is also possible.
Immunogen: Recombinant protein corresponding to amino acids 3-484 of the human Bcl-6 protein
Staining pattern: Nuclear.
Positive control: Tissue sample from lymph node or tonsil.
This antibody is designed for the specific localization of human Bcl-6 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Bcl-6 was detected by immunohistochemistry in follicular lymphomas, lymphomas, diffuse large B-cell, Burkitt's lymphomas and in Hodgkin's disease and nodular lymphocyte-predominant.