SUMMARY, EXPLANATION AND LIMITATIONS:
The molecule is a 5-transmembrane cell surface antigen with a molecular weight of 117 kD. The CD133/1 (clone AC133) antibody recognizes an epitope of the CD133 antigen. This epitope is called epitope 1 to distinguish it from another epitope (epitope 2) recognized by the clone 293C3. In the hematopoietic system, CD133 expression is restricted to a subset of CD34bright stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood. Additionally, CD133 is expressed by a small portion of CD34– cells in these tissues. The CD34+CD133+ cell population, which includes CD34+CD38– cells, was shown to be capable of repopulating NOD/SCID mice. CD133 has also been found to be expressed on circulating endothelial progenitor cells and fetal neural stem cells as well as on other tissue-specific stem cells, such as renal, prostate, and corneal stem cells. The putative murine homologue prominin is expressed on neuroepithelial and epithelial mouse cells.
Staining pattern: Cell membrane and cytoplasmic.
Positive control: Tissue sample from stem cells tumors.
This antibody is designed for the specific localization of human CD133 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.