SUMMARY, EXPLANATION AND LIMITATIONS:
CD138 / syndecan-1 protein backbone is a single chain molecule of 30.5 kDa. Five putative GAG attachment sites exist in the extracellular domain. GAG fine structure appears to reflect the cellular source of the syndecan. Expression of CD138 in human hematopoietic cells is restricted to plasma cells in normal bone marrow. Early B-cell precursors in human bone marrow are CD138 negative. CD138 is also expressed in endothelial cells, fibroblasts, keratinocytes and normal hepatocytes.
Immunogen: Tissue/cell preparation (Human): U266 cell line.
Staining pattern: Cell membrane.
Positive control: Tissue sample from tonsil.
This antibody is designed for the specific localization of human CD138 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This anti-CD138 is a good tool to highlight plasma cells and characterization of lymphomas linphoplasmatics. Some forms of Hodgkin's disease also showed positive staining with this antibody.