SUMMARY, EXPLANATION AND LIMITATIONS:
The antibody clone Carb-3 recognizes the 3-fucosyl-N-acetyl-lactosamine epitope of the CD15 antigen. It is present on the cell surface of myeloid cells, mainly on granulocytes, but not on B- or T-cells, monocytes, erythrocytes, or platelets. Additionally, the antibody reacts with Hodgkin cells and Reed-Sternberg cells. Most adenocarcinomas are positive for CD15.
Immunogen: Myelomonocytic leukaemia cells.
Staining pattern: Cell membrane.
Positive control: Tissue sample from Hodgkin’s lymphoma.
This antibody is designed for the specific localization of human CD15 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody is expressed in 90% of human neutrophil granulocytes from peripheral blood as well as a 30% -60% of circulating monocytes and thus is not present in normal lymphocytes. The CD15 antigen is also expressed in the Reed-Sternberg cells of Hodgkin's lymphoma, some leukemias and lymphomas, including mycosis fungoides. It may be useful in confirming the diagnosis of Hodgkin's disease.