SUMMARY, EXPLANATION AND LIMITATIONS:
CD99, also known as MIC-2 or single-chain Type-1 glycoprotein, is a human protein encoded by the CD99 gene. The protein has a MW of 32 kD. It is expressed on all leukocytes but highest on thymocytes, and is believed to augment T-cell adhesion and apoptosis of double-positive T-cells. It also participates in migration and activation.
Immunogen: Recombinant protein corresponding to human CD99.
Staining pattern: Cytoplasmic, cell membrane.
Positive control: Tissue sample from pancreas or testicle.
This antibody is designed for the specific localization of human CD99 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Under proper conditions of fixation and antigen retrieval, CD99 is an antigen expressed in almost 100% of Ewing sarcomas and peripheral primitive neuroectodermal tumors (PNET). However, despite such a high sensitivity labeling is not exclusively specific to this group of tumors often been described positivity in T lymphoblastic lymphomas and peripheral T-cell lymphomas, gastrointestinal stromal tumors, fibrous tumor primitive and virtually all neoplasms arising of tissues that normally express CD99.