SUMMARY, EXPLANATION AND LIMITATIONS:
CD99, or MIC2 gene product, or E2 antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors.
Immunogen: MeWo human melanoma cells.
Staining pattern: Cell membrane.
Positive control: Tissue sample from pancreas, thymus, testis or Ewing´s sarcoma.
This antibody is designed for the specific localization of human CD99 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Under proper conditions of fixation and antigen unmasking, CD99 is an antigen expressed in almost 100% of Ewing´s sarcomas and peripheral primitive neuroectodermal tumors (PNET). However, despite such a high sensitivity labeling is not exclusively specific to this group of tumors often been described positivity in T lymphoblastic lymphomas and peripheral T-cell lymphomas, gastrointestinal stromal tumors, fibrous tumor primitive and practically all of neoplasms arising of tissues that normally express CD99.