SUMMARY, EXPLANATION AND LIMITATIONS:
Cytokeratin 8 belongs to the Type B (basic) subfamily of high molecular-weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in the majority of Adenocarcinomas and Ductal Carcinomas. It is absent in Squamous Cell Carcinomas. Hepatocellular Carcinomas are de€ ned by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. Anti-Cytokeratin 8/35βH11 stains most Non-Squamous Epithelial tumors; Squamous tumors are negative for this antibody as a rule. This antibody stains Adenocarcinomas of the breast, ovary, gastrointestinal tract, thyroid, pancreas, bile duct, and salivary glands. This antibody does not react with skeletal muscle or nerve cells.
Immunogen: Cytoskeletal extract of Hep3B, a human hepatocellular carcinoma cell line.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from prostate or colon.
This antibody is designed for the specific localization of human Cytoquetarin 8 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.