SUMMARY, EXPLANATION AND LIMITATIONS:
A monoclonal antibody was raised against the major capsid protein L1 of human papilloma virus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kDa protein in cells infected with L1-vaccinia virus, and the protein was present in a predominantly nuclear location. The antibody also detects the HPV-16 L1 antigen in formalin-fixed, paraffin embedded biopsy specimens and on routine cervical smears. The antibody reacts strongly and consistently with specimens containing HPV-16 or HPV-33, but very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. A panel of p16 and Ki-67 can be used for further evaluation.
Immunogen: Capsid L1 protein of human HPV16.
Staining pattern: Nuclear.
Positive control: Tissue sample infected with HPV16.
This antibody is designed for the specific localization of viral protein of HPV16 L1 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody can be used to aid in the detection of papilloma virus antigen in a variety of skin and mucous membrane lesions, including common wart, condyloma and multiple papillomas.