SUMMARY, EXPLANATION AND LIMITATIONS:
RAS superfamily comprises about 50 genes encoding related and the GTP binding domain (G protein) involved in signal transduction. Major genes are HRAS, NRAS and KRAS. Ras GTPase proteins are bound to the membrane which binds GDP inactivated form. Receptors are activated by tyrosine kinases such as EGFR, PDGFR receptor and fibroblast growth factor. These kinases Ras-GDP converted Ras-GTP is the active form of Ras. The single amino acid substitution in the sequence of the protein can activate Ras constitutively making this a highly oncogenic protein. Such mutations prolong Ras-GTP form. The consequence of this is the activation of the transduction pathway RAF1-MAPK. Ras mutations in the protein have been found in 10-15% of tumors. A particularly high incidence has been observed in pancreatic cancer, lung and colon.
Isotype: IgG2a kappa
Immunogen: Recombinant fragment, corresponding to amino acids 54-189 of Rat Ras.
Staining pattern: Cell membrane.
Positive control: Tissue sample from skin or skin carcinoma.
This antibody is designed for the specific localization of human K-Ras using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.