SUMMARY, EXPLANATION AND LIMITATIONS:
Kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, Kappa exhibits strong staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulin. When studying B-cell neoplasms, the determination of light-chain ratios remains the centerpiece. This is sound reasoning because most B-cell Lymphomas express either kappa or lambda light chains, whereas reactive proliferations display a mixture of kappa and lambda-positive cells. If only a single light-chain type is detected, a lympho-proliferative disorder is very likely. Monoclonality is determined by a kappa-lambda ratio greater than or equal to 3:1, a lambda-kappa ratio greater than or equal to 2:1, or a monoclonal population of 75% or more of the total population.
Immunogen: Human lymphocytes stimulated.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from tonsil.
This antibody is designed for the specific localization of human kappa light chain using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody is useful for typing of FLC molecules or antibodies coupled by immunohistochemical techniques. Useful for detecting monoclonality in B cell lymphoproliferative disorders, which provides significant support diagnosis of lymphoma versus lymphoid hyperplasia or other reactive lymphadenitis, as well as in plasma cell leukemias, plasma cell dysplasias, myelomas or plasmacytomas.