SUMMARY, EXPLANATION AND LIMITATIONS:
Lambda detects surface immunoglobulin on normal and neoplastic B-cells. Lambda staining is seen in B-cell follicles of human lymphoid tissue. When studying B-cell neoplasms, the determination of light chain ratios remains the centerpiece. This is sound reasoning because most B-cell Lymphomas express either kappa or lambda light chains, whereas reactive proliferations display a mixture of kappa and lambda-positive cells. If only a single light-chain type is detected, a lymphoproliferative disorder is very likely. Monoclonality is determined by a kappa-lambda ratio greater than or equal to 3:1, a lambda-kappa ratio greater than or equal to 2:1, or a monoclonal population of 75% or moreof the total population.
Immunogen: Purified human lambda light chain.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from tonsil or lymphoid nodule.
This antibody is designed for the specific localization of human lambda light chain using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Useful for detecting monoclonality in B cell lymphoproliferative disorders, which provides significant support diagnosis of lymphoma versus lymphoid hyperplasia or other reactive lymphadenitis, as well as in plasma cell leukemias, plasma cell dysplasias, myelomas or plasmacytomas.