SUMMARY, EXPLANATION AND LIMITATIONS:
PLAP (Placental Alkaline Phosphatase) is an oncofetal antigen with a molecular weight of 60-70 kDa and belongs to a family of membrane-bound phosphatases. PLAP is detectable in syncytiothrophoblasts of the placenta and in germ cell tumours. Labelling of this enzyme is often used for identification of germ cell tumours and their discrimination from other neoplasias. Anti-PLAP antibody of clone NB10 shows cross-reactivity with human intestinal alkaline phosphatase.
Staining pattern: Cell membrane.
Positive control: Tissue sample from placenta.
This antibody is designed for the specific localization of human PLAP using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Placental alkaline phosphatase is strongly and broadly expressed in germ cell tumors, including testicular and extragonadal seminoma and dysgerminoma in ovary and is useful in identifying tumors in conjunction with other markers like M2A, OCT3/4, AP-2 and c-kit (CD117). This antibody is recommended for use in the identification of normal and tumor cells that express human placental alkaline phosphatase and type enzyme human placental alkaline phosphatase.