SUMMARY, EXPLANATION AND LIMITATIONS:
ZAP-70 is an abbrevation for Zeta-chain-associated protein kinase 70 (70 is the molecular weight in kDa). The protein is a member of the protein-tyrosine kinase family. ZAP-70 is normally expressed in T-cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 in B-cells is used as a prognostic marker in identifying different forms of Chronic Lymphocytic Leukemia (CLL). ZAP-70 protein is expressed in leukemic cells in approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases as well. ZAP-70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig-unmutated CLL cases have a poorer prognosis.
Immunogen: GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from tonsil.
This antibody is designed for the specific localization of human ZAP-70 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
It helps in the detection of CLL subtypes and studies of Severe Combined Immunodeficiency Syndrome.