SUMMARY, EXPLANATION AND LIMITATIONS:
The Tris-acetate-EDTA 10X concentrated aqueous solution. On dilution, the resultant 1X TAE Buffer will have final concentration: 40 mM Tris, 1 mM EDTA-Na2*2H2O, 20 mM acetic acid, pH 8,5.
TAE buffer is used as both a running buffer and in agarose gel. Its use in denaturing gradient gel electrophoresis methods for broad-range mutation analysis.