SUMMARY, EXPLANATION AND LIMITATIONS:
Ready-mixed buffers in powder format to be dissolved in 1 L of distilled water – safe transport, convenient and excellent quality.
On dilution, the resultant 1X Tris-Glycine will have final concentration: 25 mM Tris, 192 mM glycine, pH 8,3.
Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose. The 10X Tris-glycine buffer is diluted to 1X with methanol and water to make a solution containing 20% methanol.