SUMMARY, EXPLANATION AND LIMITATIONS:
The Tris-Glycine-SDS Buffer 10X concentrated is an aqueous solution. On dilution, the resultant 1X Tris-Glycine-SDS buffer will have final concentration: 25 mM Tris, 192 mM glycine, 0,1% SDS, pH 8,6.
Tris-glycine-SDS buffer is used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is usually used for both the anode buffer and the cathode buffer. Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.