SUMMARY, EXPLANATION AND LIMITATIONS:
Ready-mixed buffers in powder format to be dissolved in 1 L of distilled water – safe transport, convenient and excellent quality.
On dilution, the resultant 1X Tris-Glycine will have final concentration: 25 mM Tris, 192 mM glycine, 0,1% SDS, pH 8,6.
Tris-glycine-SDS buffer is used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is usually used for both the anode buffer and the cathode buffer. Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.