SUMMARY, EXPLANATION AND LIMITATIONS:
The Sodium Citrate Saline 20X concentrated is an aqueous solution. On dilution, the resultant 1X PBS buffer will have final concentration: 150 mM Sodium Chloride, 15 mM Sodium Citrate, pH 7,0.
SSC has many uses like hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer.