SUMMARY, EXPLANATION AND LIMITATIONS:
Ready-mixed buffers in powder format to be dissolved in 1 L of distilled water – safe transport, convenient and excellent quality.
On dilution, the resultant 1X SSC will have final concentration: 150 mM Sodium Chloride, 15 mM Sodium Citrate, pH 7,0.
SSC has many uses like hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer.