SUMMARY, EXPLANATION AND LIMITATIONS:
EcoNova HotTaq DNA Polymerase is a chemically modified EcoNova Taq DNA Polymerase. At ambient temperatures it is inactive, having no polymerization activity. This HotTaq DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→ 3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.
Have been purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
The EcoNova HotTaq Polymerase is a robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. This HotTaq DNA Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich).