SUMMARY, EXPLANATION AND LIMITATIONS:
EcoNova HotTaq PCR Mix Ready-to-Load is a premixed ready-to-use solution containing: all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
EcoNova HotTaq Premix contains two enzymes – EcoNova HotTaq DNA polymerase and a proofreading polymerase.
These enzymes together have both the 5' → 3' exonuclease activity as well as the 3' → 5' proofreading activity.
Reaction setup at room temperature is highly recommended for EcoNova HotTaq Premix.
We recommend using EcoNova HotTaq Premix in any PCR application that will be visualized by agarose gel electrophoresis and NovaSight DNA Stain or Ethidium bromide staining.
EcoNova HotTaq Premix Ready-to-Load is a premix for all your everyday PCR reactions, gene expression profiling, Microbial & Viral pathogen detection. This EcoNova Premix allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.