SUMMARY, EXPLANATION AND LIMITATIONS:
EcoNova M-MLV Reverse Transcriptase is a mutant of Moloney-Murine Leukemia Virus which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.
The EcoNova RTase is an enzyme for cDNA synthesis, RNA analysis by primer extension and DNA labeling.