SUMMARY, EXPLANATION AND LIMITATIONS:
Nova RedTaq Premix is a premixed solution containing everything needed for successful PCR reaction except specific primers and DNA template.
The Premix includes high-quality recombinant Nova RedTaq DNA polymerase, nucleotides and magnesium in a PCR reaction buffer.
The Premix has a special additive - the inert red dye. This makes it very suitable for standard applications. Strong red color of the mix allows user to verify adequate mixing.
Reaction products are ready for direct gel loading and the dye serves as marker for electrophoresis progress monitoring.
For the reaction set-up add the PCR premix (10 or 25 μL) to the primers, template and water for the total reaction volume of 20 or 50 μL.
To activate the Nova RedTaq Hot Thermostable DNA polymerase it should be incubated at 95-97°C for 15 minutes as a first PCR step.
This enzyme Premix allows the PCR setup at ambient temperature without nonspecific annealing and extension.
Nova RedTaq Premix is indicated for all your everyday PCR reactions, gene expression profiling, Microbial & Viral pathogen detection. This Nova Premix allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.