SUMMARY, EXPLANATION AND LIMITATIONS:
Nova Taq DNA Polymerase is a thermostable high quality recombinant enzyme that has been purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity. The enzyme is ~94 kDa by SDS-PAGE catalyzing 5'→3' synthesis of DNA. The enzyme has no detectable 3'→5' proofreading exonuclease activity. It is the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA.
Nova Taq DNA Polymerase is provided with 10fold concentrated (X) reaction buffer that contains PCR enhancers. This reaction buffer will enable or improve sub-optimal PCR caused by templates that have a high degree of secondary structure or that are GC-rich.
Nova Taq DNA Polymerase has an error rate of approximately 1 error per 2,5x105 nucleotides incorporated.
This product is sold for research purposes only.
The Taq Polymerase is a robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. This Taq DNA Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich).