SUMMARY, EXPLANATION AND LIMITATIONS:
EcoNova HotTaq EvaGreen® (registered by Biotium, Inc., Hayward, CA, USA) Probe qPCR Mix (ROX) is optimized for real-time quantitative PCR assays. The ready-to-load mix includes EcoNova HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. Only water, template and primers needs to be added.
EcoNova HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
EvaGreen® Dye: EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I (registered by Molecular Probes, Inc.), has similar spectra but much less PCR inhibition. It is extremely stable and has been shown to be nonmutagenic and noncytotoxic.
EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM.
EcoNova HotTaq qPCR Premix for Probe with ROX is a premix for all your everyday qPCR reactions with detection and quantification of DNA and cDNA targets, profiling gene expression, High Resolution Melt (HRM) Analysis, microbial detection and viral load determination.