SUMMARY, EXPLANATION AND LIMITATIONS:
EcoNova HotTaq Probe qPCR premix (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Premix contains optimized components and EcoNova HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
EcoNova HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
EcoNova HotTaq qPCR Premix for Probe with ROX is indicated for all your everyday qPCR reactions with detection and quantification of DNA and cDNA targets, profiling gene expression, High Resolution Melt (HRM) Analysis, microbial detection and viral load determination.